Human IgE protein for allergy research and isotype control
Recombinant human IgE (clone SUS-11-NBS01) produced from a human B cell hybridoma cell line established from a healthy donor (CD40 cell culture system). IgE (SUS-11) binds to the alpha-chain of the human high affinity IgE receptor and the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used for IgE monitoring in biological research samples (ELISA; FACS; W-blot, isotype control) or as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs like NBS-C BioScience mAb LE27 or a specific recombinant cross-linking agent.
Human IgE reference protein for calibrated ELISA development
Monoclonal human IgE protein to be used as reference for quantitative IgE concentration measurements in human blood serum, biological research samples, cell culture supernatants, body fluids or tissue extracts. The IgE reference standard is calibrated against the international WHO IgE reference (WHO, 2nd IRP, 75/502). It is intended to be used by researchers engaged in biological and clinical R&D in allergology and any other research field connected to investigation of IgE dependent biological systems, in particular as calibrated reference standard for human serum IgE ELISA.
Why use human IgE from NBS-C BioScience ?
Human Immunoglobulin E (hIgE)
From total immunoglobulins present in human serum IgE constitutes only a fraction of 50-300 ng/ml (compared to more than 10 mg/ml for IgG). Together with its high affinity Fc receptor, expressed on the surface of mast cells, basophils, monocytes and eosinophils IgE plays an important role in primary immune responses. In industrialized countries IgE is a pathogenic key player in the development of allergies. In general, elevated serum IgE levels indicate an increased susceptibility for IgE-mediated hypersensitivity and atopic allergic diseases such as atopic asthma, atopic dermatitis, food allergy and hay fever. Determination of total serum IgE is an important tool for differentiation between allergic diseases and other pathologies with similar clinical manifestations.