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Anti-human IgE Monoclonal Antibody (Le27)
This mouse monoclonal antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound and total IgE in human serum samples. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B – rat monoclonal anti- mouse Ck.
Applications: ELISA, FACS, W-blot, functional cellular assays (mast cell / basophil activation